3. Benchtop System Assembly¶
3.1. Assembly of Stage, Tower, and Lighting¶
Begin system assembly by removing all items from the shipping package. Confirm that the items shown in the images below. If you are missing components, please contact Resonon.
Install lighting onto the tower. Begin by sliding the T-nuts into the slots on the tower. Slide in one T-nut into each slot on the same side as the adjustable post holder.
Position the T-nuts side-by-side at the location you wish to attach your lights. (Note: You may easily adjust the lighting position later.)
Place the lighting assembly on the post such that the mounting holes line up with the T-nuts.
Attach the lighting assembly to the T-nuts using the supplied bolts.
Next, attach the post to the baseplate. Begin by removing the four bolts from the bottom of the post.
Align the post to the baseplate, then tip the baseplate up and attach using the four bolts just removed from the bottom of the post.
After tightening all four bolts, tip the tower upright.
Connect the lighting assembly to the regulated power supply.
Take the red and black banana plugs, and insert them into the red and black connectors on the regulated power supply.
Push the wire into a slot on the side of the tower.
Plug the regulated power supply into a 120 V socket and turn on to test the lighting. Turn the lights off after testing to continue with assembly.
If the lights do not turn on, turn off the red power switch and check all connections. If the lights continue to fail, contact Resonon.
When illuminating your sample with Resonon’s lighting system, allow 20 minutes for the lighting to fully stabilize.
Using the supplied USB cable, connect the stage to your computer. Plug the cable into a black USB 2.0 port, not a blue USB 3.0 port.
Plug the mini-USB connector end into the stage motor.
Next, connect the power to the stage using the provided DC power supply.
Assembly of your linear scanning stage system with stabilized lighting assembly is now complete.
3.2. Setup of Hyperspectral Imager¶
3.2.1. Objective Lens Installation¶
Resonon imaging spectrometers are designed to operate with Schneider C-mount lenses. Typically, the imager comes with a lens already installed. However, should you need to install a lens, unlock the set screw on the chrome lens collar of the Schneider lens with a 2 mm hex wrench. NOTE: Do NOT remove the screw all the way, just loosen it a few turns.
Holding onto the objective lens by the chrome collar, unscrew the black lens barrel out until there is approximately a 10mm gap between the barrel and collar.
Do NOT install the lens onto the imager if the barrel is screwed in too far as shown below.
While holding and squeezing the chrome collar, thread the objective lens onto the imager.
The objective lens is now installed.
3.2.2. Mounting the Imager¶
Screw the posts onto the bottom of your Pika imaging spectrometer. The studs in the posts are ¼”-20. Install both posts in neighboring mounting holes on the bottom of the imaging spectrometer.
Insert the posts into the adjustable post holder on the tower.
Tighten setscrew on the adjustable post holder to secure your Pika imaging spectrometer.
To adjust the height of your Pika imaging spectrometer, loosen the handle on the side of the adjustable post holder, move to your desired position, and then re-tighten the handle.
3.2.3. Connecting the Imager¶
All Pika imaging spectrometers must be interfaced to a computer for data acquisition. Depending on which Pika imaging spectrometer you have, this interface may utilize a USB or Ethernet interface. Additionally, some Pika spectrometers require external power. The image below shows interfacing with USB 3, which also provides power to the camera.
Using the provided interface cable, connect your Pika imaging spectrometer to the appropriate port on your computer, and (optionally) connect the power supply.
3.2.4. Setting Objective Lens Aperture¶
You may need to adjust the f-stop (f/#) of the objective lens. There is a range of acceptable options and you will need to choose the appropriate value based on the application. Larger f/#s provide larger depths of field, but limit the amount of light collected, whereas smaller f/#s will have smaller depths of field but exhibit better light gathering.
Changing the f/# invalidates any radiometric calibration (rad cal) of the imager, requiring re-calibration by Resonon. This is only a concern if your imager came with an Imager Calibration Pack (ICP).
To set the f/# on Schneider lenses, for example, loosen the setscrew on the objective lens itself. This will allow you to rotate the f/# collar on the objective lens, which can be read by noting the location of a small white alignment line. Align the white line with your desired f/#, then retighten the setscrew.
2.4 and larger
2.4 and larger
2.4 and larger
1.8 and larger
Initial setup is now complete. Instructions for how to collect hyperspectral images are given in the next chapter, Section 4: Basic Data Acquisition.